Methodological improvements of pyrosequencing technology

Method Development and Applications of Pyrosequencing Technology

The ability to determine nucleic acid sequences is one of the most important platforms for the detailed study of biological systems. Pyrosequencing technology is a relatively novel DNA sequencing technique with multifaceted unique characteristics, adjustable to different strategies, formats and instrumentations. The aims of this thesis were to improve the chemistry of the Pyrosequencing techniq...

Elutriation: Theoretical Considerations and Methodological Improvements

The conventional elutriation method used for separating meiofauna and sediment yields insufficiently correct quantitative results. A critical evaluation of the physical background of this method leads to the construction of a differently shaped separation funnel and to improvements allowing better controlled, gradual variations in waterflow velocity and sieve mesh sizes. Comparative tests of th...

Determining BPL Status Some Methodological Improvements

Methodological Improvements in Combining TMS and Functional MRI

....................................................................................................................................... 5 Synopsis........................................................................................................................................ 7

Mitochondrial sequence analysis for forensic identification using pyrosequencing technology.

Over recent years, requests for mtDNA analysis in the field of forensic medicine have notably increased, and the results of such analyses have proved to be very useful in forensic cases where nuclear DNA analysis cannot be performed. Traditionally, mtDNA has been analyzed by DNA sequencing of the two hypervariable regions, HVI and HVII, in the D-loop. DNA sequence analysis using the conventiona...